Mouse/Canine Integrin beta 2/CD18 Affinity Purified PAb (25 UG)【科瑞奥博】

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Mouse/Canine Integrin beta 2/CD18 Affinity Purified PAb (25 UG)

货号： AF2618-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF2618-SP
别名	959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 5% cross‑reactivity with rmIntegrin beta 1, rmIntegrin beta 4, and rmIntegrin beta 7 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Adhesion Activation: The adhesion of CD3⁺ purified mouse T cells to immobilized Recombinant Mouse ICAM‑1 Fc Chimera (Catalog # 796-IC, 10 µg/mL, 100 µL/well) was maximally enhanced (2-2.5 fold) by 1 µg/mL of the antibody.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3689 (Human); 16414 (Mouse); 309684 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 2 Gln24-Asn702 Accession # P11835
生物活性	Mouse, Canine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse Integrin beta 2 is a transmembrane glycoprotein with an 85 kDa molecular weight (predicted) and is expressed by all leukocytes. The Integrin beta chain can be found in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4) leading to different Integrin proteins. The extra cellular domains of mouse and canine Integrin beta 2 share an 80.3% aa sequence identity. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling and leukocyte trafficking.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules Adhesive Interactions in Allergy and Asthma B Cell Chemotaxis, Migration, and Adhesion Dendritic Cell Adhesion and Migration Endothelial Cell Markers and Adhesion Molecules in Angiogenesis HGF/SF Activation of Integrins Human CD Marker Products ICC / IHC Images: Integrin beta 2/CD18 Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules Myeloid Lineage Markers Platelets Regulatory T Cells (Tregs) T Cell Migration/Adhesion

参考图片

Detection of Integrin beta 2/CD18 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Mouse/Canine Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2618, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).