| 货号 | AF2446-SP |
| 别名 | AI505981; CHI3L3; Chitinase 3-like 3; ECF-L; Ym1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(2.5 µg/mL) |
| 目标/特异性 | Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 2.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 12655 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Severe Reduction in Number and Function of Peripheral T Cells Does Not Afford Protection toward Emphysema and Bronchial Remodeling Induced in Mice by Cigarette Smoke | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3 Tyr22-Tyr398 Accession # O35744 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | YM1, also known as Chitinase 3-like 3 and ECF-L, is a secreted 377 amino acid residue lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. YM1 shares homology with chitinase family proteins but lacks enzymatic activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Mouse YM1/Chitinase 3‑like 3 by Simple WesternTM. Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
