Mouse Complement Component C5a Affinity Purified PAb (25 UG)【科瑞奥博】

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Mouse Complement Component C5a Affinity Purified PAb (25 UG)

货号： AF2150-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF2150-SP
别名	C5; C5A; HE
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse Complement Component C5a in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), approximately 50% cross-reactivity with recombinant human Complement Component C5a is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Complement Component C5a-induced chemotaxis in the J774A.1 mouse reticulum cell sarcoma macrophage cell line [Zwirner, J. et al. (1998) Eur. J. Immunol. 28:1570]. The Neutralization Dose (ND₅₀) is typically 0.6-3.0 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse Complement Component C5a.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	727 (Human); 15139 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Primed CD8(+) T-cell responses to allogeneic endothelial cells are controlled by local complement activation. Authors: Raedler H, Yang M, Lalli PN Am. J. Transplant., 2009;9(8):1784-95. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Decay accelerating factor can control T cell differentiation into IFN-gamma-producing effector cells via regulating local C5a-induced IL-12 production. Authors: Lalli PN, Strainic MG, Lin F, Medof ME, Heeger PS J. Immunol., 2007;179(9):5793-802. Species: Mouse Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse Complement Component C5a Asn679-Arg755 Accession # P06684
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a non-signaling GPCR termed C5L2 whose function is yet to be determined (5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705. Okinaga, S. et al. (2003) Biochemistry 42:9406.

参考图片

Chemotaxis Induced by Complement Component C5a and Neutralization by Mouse Complement Component C5a Antibody. Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) chemoattracts the J774A.1 mouse reticulum cell sarcoma macrophage cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Complement Component C5a (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Complement Component C5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2150). The ND₅₀ is typically 0.6-3.0 µg/mL.