| 货号 | AF8280-SP |
| 别名 | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6; CPAMD6; pregnancy zone protein; pregnancy-zone protein; PZP |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) |
| 目标/特异性 | Detects human Pregnancy Zone Protein/PZP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human A2ML1 is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5858 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human Pregnancy Zone Protein/PZP Thr26-Val1482 Accession # P20742 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Pregnancy zone protein (PZP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6) is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins. Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also this protein is found in plasma. Over aa 26-1482, human PZP is 56% aa identical to mouse PZP. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Pregnancy Zone Protein/PZP by Western Blot. Western blot shows human pregnancy serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Pregnancy Zone Protein/PZP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
