| 货号 | AF8126-SP |
| 别名 | complement component 9; complement component C9 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human Complement Component C9 in directs ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 735 (Human); 12279 (Mouse); 117512 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived human Complement Component C9 Gln22-Lys559 (predicted) Accession # P02748 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Complement Component C9 by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Complement Component C9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
