| 货号 | AF7927-SP |
| 别名 | AADAT; aminoadipate aminotransferase; EC 2.6.1.39,2-aminoadipate aminotransferase; EC 2.6.1.7,2-aminoadipate transaminase; KAT/AadAT; KAT2; KAT2mitochondrial; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase II; L kynurenine/alpha aminoadipate aminotransferase | 全称 | alpha-Aminoadipate Aminotransferase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human alpha -Aminoadipate Aminotransferase in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51166 (Human); 23923 (Mouse); 29416 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human alpha -Aminoadipate Aminotransferase Met1-Leu425 Accession # Q8N5Z0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Alpha-Aminoadipate Aminotransferase (AADAT, KAT2 and Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial) is a 47 kDa (predicted) mitochondrial member of the class-I pyridoxal-phosphate-dependent aminotransferase family of proteins. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. Human ADDAT is predominantly expressed in the liver, it is also found in heart, brain, kidney, pancreas, prostate, testis and ovary. Over aa 1-425, human AADAT shares 73% aa sequence identity with mouse ADDAT. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human alpha -Aminoadipate Aminotransferase by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human alpha -Aminoadipate Aminotransferase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7927) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha -Aminoadipate Aminotransferase at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
