| 货号 | AF7830-SP |
| 别名 | autosomal 2; NADPH oxidase activator 2; NCF2; NCF-2; neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal2); neutrophil cytosolic factor 2; NOXA2; NOXA2FLJ93058; p67phox; p67-phox |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human p67phox in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4688 (Human); 17970 (Mouse); 364018 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human p67phox Lys355-Val526 (His389Gln) Accession # P19878 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | P67phox (67 kDa phagocyte oxidase; also NCF2 and NADPH oxidase activator 2) is a 67-70 kDa cytosolic member of the NCF2/NOXA1 family of proteins. It is expressed in multiple cell types, including B cells, endothelial cells, phagocytic cells such as neutrophils and macrophages and, to some degree, fibroblasts. Upon phagocytosis, phagocytes generate superoxide for the purpose of killing ingested microbes. This occurs through the formation and activation of a complex termed NADPH oxidase, which consists of membrane-associated cytochrome b558, plus Rac GTPase and three SH3 domain-containing components, p47phox and a p67phox:p40-phox dimer. Human p67phox is 526 amino acids (aa) in length. It contains two TPR repeats that participate in scaffold formation (aa 11-98), an SH3 domain (aa 240-299), an OPR motif that regulates PB1 interactions (aa 351-429), and a C-terminal SH3 domain (aa 457-516). There is one splice variant that shows a deletion of aa 231-293, and multiple point mutations along the length of the molecule. Over aa 355-526, human p67phox shares 76% aa identity with mouse p67phox. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human p67phox by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and human immature dendritic cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human p67phox Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7830) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for p67phox at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
