| 货号 | AF7817-SP |
| 别名 | CCBL1; cysteine conjugate-beta lyase, cytoplasmic; cysteine conjugate-beta lyase; cytoplasmic (glutamine transaminase K; Cysteine-S-conjugate beta-lyase; EC 2.6.1.64; EC 2.6.1.7; EC 4.4.1.13; FLJ95217; Glutamine Transaminase K; glutamine-phenylpyruvate aminotransferase; Glutamine--phenylpyruvate transaminase; GTKMGC29624; KAT1; KATI; KATIbeta-lysase, kidney; Kyneurenine Aminotransferase I; kyneurenine aminotransferase; kyneurenine aminotransferase); Kynurenine aminotransferase I; kynurenine--oxoglutarate transaminase 1; Kynurenine--oxoglutarate transaminase I |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Cysteine Conjugate beta -Lyase/CCBL1 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 883 (Human); 70266 (Mouse); 311844 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf21-derived recombinant human Cysteine Conjugate beta -Lyase/CCBL1 Thr182-Leu422 Accession # Q16773 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CCBL1 (Cysteine Conjugate beta -Lyase 1; also GTK and KAT-I) is a 90-120 kDa homodimeric member of the Class I Pyridoxyl-phosphate-dependent aminotransferase family of enzymes. It is widely expressed, being found in fibroblasts, renal epithelium, hepatocytes, neurons and astrocytes. CCBL1 has multiple activities, including converting prodrugs into active formulations, generating kynurenic acid from kynurenine, thereby antagonizing NMDA receptors (but not likely presynaptic alpha 7 AChRs), and blocking the release of proinflammatory cytokines by microglia via GPCR-35 antagonism. Human CCBL1 is 422 amino acids (aa) in length, and contains one aspartate aminotransferase-like region (aa 31-417). There are three potential splice variants, one that shows a deletion of aa 68-117, a second that shows a deletion of aa 251-422, and a third that contains a 21 aa substitution for aa 230-250. While the 422 aa isoform is considered cytosolic, there is the potential for a 34 aa N-terminal extension that would act as a mitochondrial targeting sequence. Such a sequence is reported for rat and nonhuman primate. Over aa 182-422, human CCBL1 shares 82% aa sequence identity with mouse CCBL1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human, Mouse, and Rat Cysteine Conjugate beta -Lyase/CCBL1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Cysteine Conjugate beta -Lyase/CCBL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cysteine Conjugate beta -Lyase/CCBL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
