| 货号 | AF7788-SP |
| 别名 | CYP8; CYP8A1; CYP8A1PTGI; CYP8prostacyclin synthase; EC 5.3.99.4; MGC126858; MGC126860; PGIS; PGIScytochrome P450, family 8, subfamily A, polypeptide 1; Prostacyclin Synthase; prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Prostaglandin I2 Synthase in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5740 (Human); 19223 (Mouse); 25527 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Prostaglandin I2 Synthase Arg21-Pro500 Accession # Q16647 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma. Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Prostaglandin I2 Synthase by Western Blot. Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, human ovary tissue, human prostate tissue, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti‑Human Prostaglandin I2 Synthase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
