| 货号 | AF7787-SP |
| 别名 | Complexin-1/2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Complexin-1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Complexin-1 Met1-Lys134 Accession # O14810 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Complexin-1 (CPX I; also synaphin-2) is a 17-18 kDa, cytosolic protein that belongs to the complexin/synaphin family, complexin-1/-2 subfamily of molecules. It is expressed in select neurons and pancreatic beta -cells, and serves as a block against premature neurotransmitter/insulin granule release. Both Complexin-1 and Complexin-2 bind to the SNARE complex that contains SNAP25, VAMP2 and STX1A, generating oligomers of SNARE complexes that are optimally oriented relative to the positions of vesicle and plasma membranes. This effectively places synaptic vesicles in an optimal configuration, allowing for a coordinated, uniform fusion and release upon appropriate stimulus. Typically, complexin-1 is described as a clamp that blocks (vesicle) fusion, and the stimulus that overcomes it is one that induces an influx of synaptotagmin-detectable calcium. Human complexin-1 is 134 amino acids (aa) in length. It contains one coiled-coil domain (aa 29-69) that incorporates part of a SNARE complex interaction sequence (aa 48-70). Full-length human complexin-1 shares 97% aa sequence identity with mouse complexin-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Complexin-1/2 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue, mouse brain tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Complexin-1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7787) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Complexin-1/2 at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Complexin-1/2 in Rat Hippocampal Neurons. Complexin-1/2 was detected in immersion fixed rat hippocampal neurons (14 days in vitro) using Goat Anti-Human/ Mouse/Rat Complexin-1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7787) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
