| 货号 | AF7457-SP |
| 别名 | carboxypeptidase M; CPM; EC 3.4.17; EC 3.4.17.12 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Carboxypeptidase M in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CPE is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1368 (Human); 70574 (Mouse); 314855 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Carboxypeptidase M Leu18-Ser423 Accession # P14384 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CPM (Carboxypeptidase M) is a 50‑65 kDa monomer that belongs to the regulatory CPN/E subfamily, peptidase M14 family of enzymes. It is widely expressed, being found on macrophages, fibroblasts, endothelial cells, oligodendrocytes and Schwann cells, dendritic cells, osteoblasts and bronchial epithelium. CPM is a GPI‑linked glycoprotein that is best known as a peptidase that cleaves basic amino acids (aa) from the carboxyterminal of a number of peptides, including EGF and bradykinin. It is also known to bind to apparent substrates and undergo a conformational change that links it with the kinin B1 GPCR, initiating signal transduction. Mature human CPM is 406 aa in length (aa 18-423). It contains one large enzymatic region (aa 19-310) and two critical glutamic acid residues at Glu260 and Glu264. Like many GPI‑linked proteins, CPM undergoes solubilization and is reportedly found in urine and amniotic fluid. Over aa 18-423 (mature CPM), human CPM shares 86% aa sequence identity with mouse CPM. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Carboxypeptidase M by Western Blot. Western blot shows lysates of human placenta tissue and human adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carboxypeptidase M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxypeptidase M at approximately 58-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Carboxypeptidase M in Human Placenta. Carboxypeptidase M was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Carboxypeptidase M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7457) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
