| 货号 | AF7387-SP |
| 别名 | glutamate receptor, metabotropic 4; GPRC1D; GPRC1Dmetabotropic glutamate receptor 4; GRM4; MGC177594; mGlu4; mGluR4; MGLUR4glutamate metabotropic receptor 4 | 全称 | Metabotropic Glutamate Receptor 4 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human mGluR4 in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) mGluR7 and rhmGluR8 is observed, and less than 8% cross-reactivity with rhmGluR3 is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2914 (Human); 268934 (Mouse); 24417 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human mGluR4 Lys33-Ser518 Accession # Q14833 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Metabotrophic glutamate receptor 4 (mGluR4) is a 95-110 kDa Group III mGluR member of the Class C, GPCR 3 family of proteins. The mGluR family of receptors are 7-transmembrane (TM), non-ion channel glutamate-binding molecules that are linked to adenylcyclase. mGluR4 is widely expressed, being found on/in multiple cell types, including colonic, breast, urinary and skin epithelium. It is also found on neurons where it is located presynaptically and regulates neurotransmitter release. In general, mGluR4 activation results in a slowing of either glutamine or GABA release. However, in discrete areas, it actually facilitates glutamine release. Mature human mGluR4 is an 880 amino acid (aa) 7-TM molecule (aa 33-912). It contains a 555 aa N-terminal extracellular region (aa 33-587), plus a 65 aa C-terminal cytoplasmic domain. mGluR4 potentially forms homodimers, and is reported to heterodimerize with both mGluR2 and mGluR8. Based on rodent, there is one 68 kDa isoform that is found in taste buds and shows an alternative start site at Met309. Two additional potential splice variants contain a second alternative start site at Met209, and an Asp substitution for aa 343-390, respectively. It is uncertain if human possesses a mGluR4b found in rodent. Over aa 1-518, human mGluR4 shares 96% aa identity with mouse mGluR4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of mGluR4 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Sheep Anti-Human mGluR4 Affinity-purified Polyclonal Antibody (Catalog # AF7387, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). | |
mGluR4 in Human Endothelial Cells. mGluR4 was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using Sheep Anti-Human mGluR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7387) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
