| 货号 | AF7384-SP |
| 别名 | Alpha-fetoprotein enhancer-binding protein; AT motif-binding factor 1; AT motif-binding factor; ATBF1alpha-fetoprotein enhancer binding protein; AT-binding transcription factor 1ATBT; ATBT; ZFH3; ZFH-3; ZFHX3; zinc finger homeobox 3; zinc finger homeobox protein 3; Zinc finger homeodomain protein 3; ZNF927 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ATBF1/ZFHX3 in direct ELISAs. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 463 (Human); 11906 (Mouse); 307829 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ATBF1/ZFHX3 His3608-Leu3703 Accession # Q15911 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ATBF1 (Adenine-Thymine motif Binding Factor 1; also ZFHX3) is a 400 kDa highly modular protein that contains motifs found in multiple transcription factor families. It is expressed by a variety of cells, both normal and malignant, including postmitotic neurons, hepatocytes, mammary epithelium and gastric carcinoma cells. ATBF1 is both cytoplasmic and nuclear, and its localization has pronounced effects on the cell cycle. Cytoplasmic ATBF1 is associated with cell proliferation, while nuclear ATBF1 appears to promote cell differentiation. Human ATBF1-A is 3703 amino acids (aa) in length. It contains seventeen consecutive C2H2-type Zn finger domains interspersed with short poly-Glu and poly-Ala segments (aa 282-2006), followed by two homeobox domains (aa 2146-2301), two additional C2H2 Zn finger domains (aa 2328-2552) and two homeobox plus three intermixed Zn finger domains. There are at least six utilized Ser phosphorylation sites. Two isoform variants have been reported. The first is termed ATBF1-B. It has a predicted MW of 306 kDa and appears following the use of an alternative start site at Met915. When the 914 aa N‑terminus is present (as in ATBF1-A), ATBF1 shows transcriptional repression; when it is absent (as in ATBF1-B), ATBF1 acts as a transcriptional activator. The second isoform variant contains a 41 aa substitution for aa 1150-3703. Over aa 3608-3703, human ATBF1 shares 98% aa sequence identity with mouse ATBF1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ATBF1/ZFHX3 in MCF‑7 Human Cell Line. ATBF1/ZFHX3 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human ATBF1/ZFHX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7384) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
