| 货号 | AF7014-SP |
| 别名 | beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFNARB; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2 | 全称 | Interferon alpha/beta Receptor 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human IFN-alpha / beta R2 C-Terminus in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IFN-alpha / beta R2a, rhIFN-alpha / beta R alpha, and recombinant mouse IFN-alpha / beta R2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3455 (Human); 15976 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IFN-alpha / beta R2 C-Terminus Ser351-Met514 Accession # P48551 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | IFN-alpha / beta R2 (Interferon alpha/beta receptor 2/IFNAR2; also IFN alpha binding protein) is a 90-102 kDa member of the class II cytokine receptor family of molecules. It is expressed on hematopoietic cells, including T cells, NK cells, B cells and monocytes. It serves as a ligand binding subunit for type I IFN, and generates a functional IFN receptor by complexing with 125 kDa IFNAR1. Multiple type I IFNs signal through this heterodimeric receptor with distinct outcomes. This may be attributable to unique structural conformations created by the Type I IFN:IFNAR1 interaction, or the use of a truncated isoform of IFNAR2. Mature human IFNAR2 is a 489 amino acid (aa) type I transmembrane glycoprotein (aa 27-515). It contains a 217 aa extracellular region (aa 27-243) that contains an Ig-like domain (aa 39-118), a fibronectin type III domain, and a 251 aa cytoplasmic tail. There are two isoform variants. The first is 50-55 kDa in size and contains a 51 aa substitution for aa 281-515. The second is a 40 kDa soluble form that shows a two aa substitution for aa 238-515. IFNAR2 also undergoes proteolysis. Cleavage of the 92 kDa isoform generates a 58 kDa membrane-embedded stub that can be further cleaved into 27-35 kDa fragments that undergo nuclear translocation. Over aa 351-514. human IFNAR2 shares 48% aa identity with mouse IFNAR2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human IFN‑ alpha / beta R2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, untreated (-) or treated with PMA. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IFN‑ alpha / beta R2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7014) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN‑ alpha / beta R2 C-terminus at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
