| 货号 | AF6948-SP |
| 别名 | CHC22; clathrin heavy chain 2; clathrin heavy chain on chromosome 22; clathrin, heavy chain-like 1; Clathrin, heavy polypeptide D; CLH-22; CLTCL; CLTCL1; CLTD; heavy polypeptide-like 1 | 全称 | Clathrin Heavy Chain on Chromosome 22 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Clathrin Heavy Chain 2/CHC22 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC17 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8218 (Human); 98970 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Clathrin Heavy Chain 2/CHC22 Trp1521-Glu1640 Accession # P53675 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CHC22 (clathrin heavy chain on chromosome 22; also Clathrin Heavy Chain 2, CHL-22, CLTCL1, and CLTD) is a 170-190 kDa member of the clathrin heavy chain family of molecules. It is expressed in striated (skeletal and cardiac) muscle and adipocytes. CHC22 appears to participate in glucose transport by interacting with GLUT4 and GGA2, thus contributing to the formation of the GLUT4 storage compartment. CHC22 forms homotrimers, but does not interact with clathrin light chains. Human clathrin heavy chain 2/CHC22 is 1640 amino acids (aa) in length. It contains an N-terminal domain that mediates protein-protein interactions (aa 2-479), a flexible linker region (aa 480-523), a distal segment (aa 524-634), a "proximal" region (aa 639-1640), and a trimerization domain that mediates the formation of a non‑covalent structure (aa 1551-1640). Multiple utilized phosphorylation and acetylation sites exist. There are three potential splice forms. One possesses a seven aa substitution for aa 1620-1640, a second shows a deletion of aa 1479-1535, and a third contains a combination of the afore-mentioned variations. Over aa 1521‑1640, human CHC22 shares 60% aa identity with canine CHC22 and 73% aa identity with human CHC17. In rodent, CHC22 is not expressed as it is represented by a pseudogene. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Clathrin Heavy Chain 2/CHC22 by Western Blot. Western blot shows lysates of human testis tissue and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 2/CHC22 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Clathrin Heavy Chain 2/CHC22 in Human Mesenchymal Stem Cells. Clathrin Heavy Chain 2/CHC22 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Sheep Anti-Human Clathrin Heavy Chain 2/CHC22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6948) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
