| 货号 | AF6784-SP |
| 别名 | CHC; CHC17; clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); clathrin, heavy chain; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH17; CLH-17; CLTC; CLTCL2; Hc; KIAA0034 | 全称 | Clathrin Heavy Chain on Chromosome 17 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Clathrin Heavy Chain 1/CHC17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC-22 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1213 (Human); 67300 (Mouse); 54241 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Clathrin Heavy Chain 1/CHC17 Trp1521-Met1675 Accession # Q00610 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Clathrin Heavy Chain 1/CHC17 (clathrin heavy chain 17; also CHC, CLTCL2 and CLTC) is a 170‑180 kDa member of the clathrin heavy chain family of molecules. It is widely expressed, and serves at least two functions. First, it interacts with TACC3 and chTOG to form a complex that crosslinks microtubules making up K‑fibers, and second, three Clathrin Heavy Chain 1/CHC17 molecules form a triskelion (Greek for "3 legged") structure with three clathrin light chains to create the polyhedral coat of coated pits. Human Clathrin Heavy Chain 1/CHC17 is 1675 amino acids (aa) in length. It contains an N‑terminal domain that mediates protein‑protein interactions (aa 2‑479), a flexible linker region (aa 480‑523), a distal segment (aa 524‑638), a proximal region that interacts noncovalently with one CHC light chain (aa 639‑1549), and a trimerization domain that mediates the formation of a noncovalent triskelion structure (aa 1550‑1675). Although the predicted MW of Clathrin Heavy Chain 1/CHC17 is 191 kDa, it runs anomalously at 180 kDa in SDS‑PAGE. Multiple utilized phosphorylation and acetylation sites exist. There are multiple potential splice forms. One shows a four aa insertion after Lys83, a second shows a seven aa insertion after Asp1611, and a third contains a four aa substitution for aa 1636‑1675. Over aa 1521‑1675, human and mouse Clathrin Heavy Chain 1/CHC17 are identical in aa sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Clathrin Heavy Chain 1/ CHC17 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Clathrin Heavy Chain 1/CHC17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6784) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Clathrin Heavy Chain 1/CHC17 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Clathrin Heavy Chain 1/CHC17 in HeLa Human Cell Line. Clathrin Heavy Chain 1/CHC17 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Clathrin Heavy Chain 1/CHC17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6784) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Clathrin coated vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
