| 货号 | AF6526-SP |
| 别名 | EC 2.1.1.20; glycine N-methyltransferase; GNMT |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Glycine N-methyltransferase/GNMT in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27232 (Human); 14711 (Mouse); 25134 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Glycine N-methyltransferase/GNMT Met1-Asp295 Accession # Q14749 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Glycine N-methyltransferase/GNMT by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6526) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
