Human Adenosylhomocysteinease/AHCY Affinity Purified PAb (25 UG)【科瑞奥博】

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Human Adenosylhomocysteinease/AHCY Affinity Purified PAb (25 UG)

货号： AF6466-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF6466-SP
别名	adenosylhomocysteinase; AdoHcyase; AHCY; EC 3.3.1.1; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; SAHH; SAHHadoHcyase
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	191 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inhibition of cellular methyltransferases promotes endothelial cell activation by suppressing glutathione peroxidase 1 protein expression. Authors: Barroso M, Florindo C, Kalwa H, Silva Z, Turanov A, Carlson B, de Almeida I, Blom H, Gladyshev V, Hatfield D, Michel T, Castro R, Loscalzo J, Handy D J Biol Chem, 2014;289(22):15350-62. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Adenosylhomocysteinase/AHCY Ser2-Tyr432 Accession # P23526
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Human S‑Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co‑factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S‑adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S‑adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S‑adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101. Takata, Y. et al. (2002) J. Biol. Chem. 277:22670. Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942. Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234. Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347.

参考图片

Detection of Human Adenosylhomocysteinase/AHCY by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.