| 货号 | AF6419-SP |
| 别名 | netrin 1; netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Netrin-1 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9423 (Human); 18208 (Mouse); 396389 (Chicken) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC) | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Netrin-1 Val22-Ala604 Accession # O95631 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Netrin-1 (from Sanskrit netr meaning "to guide"; also known as Epididymis tissue protein Li 131P) is a 66-76 kDa secreted member of the laminin superfamily of molecules. It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia. Netrin-1 has multiple receptors, including beta 4-integrins, DCC, DSCAM and UNC5A-thru-D. Its effects are context specific. For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion. In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis. Mature human netrin-1 is 580 amino acids (aa) in length (aa 25-604). It contains an N-terminal laminin domain (aa 47-284), three EGF-like domains (aa 285-453) and a C-terminal cell-interaction netrin-like domain (aa 472-601). Over aa 22-604, human netrin-1 shares 99% aa sequence identity with mouse netrin-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Netrin‑1 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Netrin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6419) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Netrin‑1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
