| 货号 | AF628-SP |
| 别名 | chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots. In direct ELISAs and Western blots, a preference for the 68 aa residue isoform over the 92 aa residue isoform of human CCL15/LKN-1 is shown. Additionally, in direct ELISAs, no cross-reactivity with ____________ is observed. Neutralizes the biological activity of the 68 aa isoform of recombinant human (rh) CCL15/LKN-1. It will also neutralize the biological activity of the 92 aa residue isoform of rhCCL15/LKN-1 using a 2-fold higher Ig concentration. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL15/MIP‑1δ-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 15 ng/mL Recombinant Human CCL15/MIP‑1δ 68 aa. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6359 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CCL15/MIP-1δ Ser46-Ile113 Accession # Q16663 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Macrophage inflammatory protein 1 delta (MIP-1 delta), also named Leukotactin-1 (LKN-1), MIP-5, HCC-2, and NCC-3, together with mouse C10, mouse MIP-1 gamma and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in MIP-1 delta/LKN-1 form a third disulfide bond. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL15/MIP‑1δ and Neutralization by Human CCL15/MIP‑1δ Antibody. Recombinant Human CCL15/MIP‑1δ (Catalog # 628‑LK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP‑1δ (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP‑1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF628). The ND50 is typically 0.5-2.5 µg/mL. |
