| 货号 | AF5980-SP |
| 别名 | complement factor H-related protein 4; CFHL4; CFHR4; complement factor H-related 4; FHR4; FHR-4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) CFHR1, rhCFHR2, and rhCFHR5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10877 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4 Glu20-Glu331 Accession # Q92496 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1(FHL-1) and five factor H-related proteins (CFHR-1 to -5). Members of the H-related protein family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulators of complement activation (RCA) gene clusters. Complement factor H-related protein 1 (CFHR1) is a 43 kDa, secreted member of the factor H family of glycoproteins. It is produced by hepatocytes and circulates as two differentially glycosylated isoforms (37 kDa and 43 kDa). Complement Factor H-related 2 (CFHR2) is synthesized in the liver and secreted into plasma. It may be involved in complement regulation. CFHR2 can also be associated with lipoproteins and may play a role in lipid metabolism. CFHR-5 has been identified initially as a universal component of complement deposits, and detected in glomerular immune deposits. The pattern of deposits is similar to other complement components, suggesting that CFHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. CFHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association. Weak factor I-dependent cofactor activity for C3b cleavage has also been observed. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot. Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Complement Factor H-related 4/CFHR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5980) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H-related 4/CFHR4 at approximately XXX kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group XXX. |
