| 货号 | AF5969-SP |
| 别名 | CBG; CBGL1; CBGL1GLUC; cytosolic beta-glucosidase; Cytosolic beta-glucosidase-like protein 1; EC 3.2.1.21; GBA3; GLUC; glucosidase, beta, acid 3 (cytosolic); klotho-related protein; KLrP; MGC104276; MGC126878 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Cytosolic beta ‑Glucosidase/GBA3 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation |
| 来源 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57733 (Human); 289687 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta ‑Glucosidase/GBA3 Accession # Q9H227 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide. GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides. GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides. GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism. At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cytosolic beta ‑Glucosidase/GBA3 by Western Blot Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic beta ‑Glucosidase/GBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5969) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic beta ‑Glucosidase/GBA3 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
