| 货号 | AF5944-SP |
| 别名 | EC 3.1.2.6; GLO2hydroxyacyl glutathione hydrolase; Glx II; GLX2; GLXII; Glyoxalase II; HAGH; HAGH1GLX2; hydroxyacylglutathione hydrolase; hydroxyacylglutathione hydrolase, mitochondrial; hydroxyacylglutathione hydroxylase |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Glyoxalase II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Glyoxalase I is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3029 (Human); 14651 (Mouse); 24439 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Glyoxalase II Met49-Ile254 Accession # Q16775 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The glyoxalase system consists of two enzymes, Glyoxalase I and Glyoxalase II. The purpose of the glyoxalase system is to detoxify alpha-ketoaldehydes, particularly methylglyoxal, a by-product of glycolysis. Glyoxalase II catalyzes the hydrolysis of S-lactoylglutathione, the product of the glyoxalase I reaction, to glutathione and lactate. Glyoxalase II exists as two isoforms, one cytosolic and one mitochondrial. The enzyme is believed to be important for the response of cells to stress. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Glyoxalase II by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, human liver, human kidney, mouse liver, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Glyoxalase II at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
