| 货号 | AF5936-SP |
| 别名 | AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); autoimmune regulator; PGA1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human AIRE in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 326 (Human); 11634 (Mouse); 294328 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human AIRE Ser476-Ser545 Accession # O43918 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AIRE (Autoimmune Regulator; also APECED) is a 58‑60 kDa member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545 amino acids (aa) in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa 1‑105), an NLS (aa 131‑133), a SAND domain (aa 181‑280), and two Zn-finger PHD domains (aa 296‑343 and 434‑475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa 437‑467, a 95 aa substitution for aa 1‑292, and a 75 aa substitution for aa 377‑545. Over aa 476‑545, human AIRE shares 71% aa identity with mouse AIRE. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Autoimmune Regulator/AIRE by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Human Autoimmune Regulator/AIRE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5936) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Autoimmune Regulator/AIRE at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
