货号 | AF5805-SP |
别名 | CRF; CRH; ProCorticoliberin; Pro-Corticoliberin/Pro-CRH | 全称 | Pro-Corticotropin-releasing Hormone |
反应种属 | Human |
应用 | Western Blot(1 µg/mL) |
目标/特异性 | Detects human Pro‑Corticoliberin/Pro‑CRH in direct ELISAs and Western blots. |
使用方法 | Western Blot: 1 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 1392 (Human); 12918 (Mouse); 81648 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human Pro‑Corticoliberin/Pro‑CRH Leu25-Lys196 Accession # P06850 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot. Western blot shows lysates of human cerebellum and hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro‑Corticoliberin/Pro‑CRH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro‑Corticoliberin/Pro‑CRH at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |