| 货号 | AF5760-SP |
| 别名 | EC 2.3.2.13; Fibrinoligase; hTGP; Prostate transglutaminase; Prostate-specific transglutaminase; protein-glutamine gamma-glutamyltransferase 4; TG(P); TGase P; TGase-4; TGM4; TGP; TGPFLJ26776; transglutaminase 4 (prostate); Transglutaminase P; transglutaminase-4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Transglutaminase 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Transglutaminase 2, rhTransglutaminase 3, rhTransglutaminase 7, and recombinant mouse Transglutaminase 2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7047 (Human); 331046 (Mouse); 64679 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 4 Met2-Lys684 Accession # AAC50516 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77 kDa member of the papain-like family of transglutaminases. It is expressed by various tumor cell lines plus prostatic epithelium, and may participate in semen protein modification. TGM4, like other TGMs, can apparently mediate transamidation, plus esterification reactions. Human TGM4 is 684 amino acids (aa) in length. Although it does not contain a signal sequence, based on rodent, it is likely secreted as a homodimer. TGM4 contains an active enzyme site between aa 263‑353, and shows a C-terminal Ig-like domain (aa 587‑684). The molecule has a requirement for divalent calcium. There are two potential splice variants. One shows a 45 aa insertion after Leu8, while another shows a 12 aa substitution for aa 1‑80. Human full‑length TGM4 shares only 54% aa identity with mouse TGM4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Transglutaminase 4/TGM4 in Human Prostate. Transglutaminase 4/TGM4 was detected in paraffin-embedded sections of human prostate using Sheep Anti-Human Transglutaminase 4/TGM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5760) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Transglutaminase 4/TGM4 in Human Prostate. Transglutaminase 4/TGM4 was detected in immersion fixed paraffin-embedded sections of human prostate using Sheep Anti-Human Transglutaminase 4/TGM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5760) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Transglutaminase 4/TGM4 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Transglutaminase 4/TGM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 4/TGM4 at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
