| 货号 | AF5724-SP |
| 别名 | ACR1; ACR1Peroxisomal antioxidant enzyme; Alu corepressor 1; Alu co-repressor 1; Antioxidant enzyme B166; AOEB166; AOEB166Peroxiredoxin V; AOPP; B166; EC 1.11.1.15; Liver tissue 2D-page spot 71B; MGC117264; MGC142283; MGC142285; peroxiredoxin 5; peroxiredoxin-5, mitochondrial; PLPThioredoxin peroxidase PMP20; PMP20; PRDX5; PRDX6; PRXV; prx-V; SBBI10; Thioredoxin reductase; TPx type VI |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat Peroxiredoxin 5 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 25824 (Human); 54683 (Mouse); 113898 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Peroxiredoxin 5 Met1-Leu210 Accession # P99029 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54‑63, and a peroxisome targeting motif at aa 208‑210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17 kDa in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83‑102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Peroxiredoxin 5 by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Peroxiredoxin 5 in A549 Human Cell Line. Peroxiredoxin 5 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Peroxiredoxin 5 in A549 Human Cell Line. Peroxiredoxin 5 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5724) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (mitochondria). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
