| 货号 | AF5614-SP |
| 别名 | neuronal olfactomedin related ER localized protein 2; NOE2; NOE2noelin 2; NOELIN2; Noelin-2; NOELIN2_V1; olfactomedin 2; olfactomedin-2; OlfC; OLFM2; OM2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Olfactomedin‑2/Noelin‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse (rm) Olfactomedin‑1 is observed and less than 5% cross-reactivity with recombinant human Olfactomedin‑4 and rmOlfactomedin‑L3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 93145 (Human); 244723 (Mouse); 313783 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin‑2/Noelin‑2 Gln21-Pro454 Accession # O95897 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1‑3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58‑85 and 136‑193) and an Olfactomedin domain (aa 194‑446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Olfactomedin‑2/Noelin‑2 by Western Blot. Western blot shows lysates of human cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Olfactomedin‑2/Noelin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Olfactomedin‑2/ |
