| 货号 | AF5467-SP |
| 别名 | I100; ileal dipeptidylpeptidase; NAALADase L; NAALADASEL; NAALADase-like 1; NAALADL1 N-acetylated alpha-linked acidic dipeptidase-like 1; NAALADL1 | 全称 | N-Acetylated Alpha-Linked Acidic Dipeptidase-like 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human NAALADase-like 1/NAALADL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) NAALADase I, recombinant mouse NAALADase II, and rhNAALADase II is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10004 (Human); 381204 (Mouse); 83568 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-like 1/NAALADL1 Ala32-Leu740 Accession # NP_005459 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NAALADase-like1, also known as NAALADL1, (N-acetylated alpha -linked acidic dipeptidase like 1; also Ileal dipeptidylpeptidase) is a 100-115 kDa member of the peptidase M28 family of enzymes. It is found in the brush border of Ileal epithelium and demonstrates serine protease activity. Human NAALADL1 is a type II transmembrane glycoprotein that is 740 amino acids (aa) in length. It contains an extended extracellular domain (aa 29‑740) that shows a protease-associated region (aa 109‑335) with key catalytic residues at Ser617, Asp657 and His687. Multiple splice variants exist. Two show deletions of aa 161‑201 and 296‑330, respectively, while a third shows a 51 aa insertion after Pro359. Three show 18 to 42 aa substitutions after Pro502, with premature truncations after Ile522, Thr543 and Leu579. A final isoform shows a 17 aa substitution for aa 619‑740. Over aa 32‑740, human NAALADL1 shares 81% aa identity with mouse NAALADL1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human NAALADase-like 1/NAALADL1 by Western Blot. Western blot shows lysates of human small intestine. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAALADase‑like 1/NAALADL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAALADase‑like 1/NAALADL1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
