| 货号 | AF5460-SP |
| 别名 | Antioxidant enzyme AOE372; AOE372; AOE37-2PRX-4; EC 1.11.1.15; peroxiredoxin 4; Peroxiredoxin IV; peroxiredoxin-4; PRDX4; Prx4; prx-4; prx-IV; thioredoxin peroxidase (antioxidant enzyme); Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat Peroxiredoxin 4 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 0.3-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10549 (Human); 53381 (Mouse); 85274 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Peroxiredoxin 4 Met1-Asn271 Accession # Q13162 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 30-32 kDa, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts p53‑mediated generation of ROS, and regulates NF kappa B via I kappa B-alpha. Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region (aa 20‑30) and a 159 thioredoxin domain (aa 79‑237). There is one catalytic cysteine at Cys124. Over amino acids 1‑271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Peroxiredoxin 4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Peroxiredoxin 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 4 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
