Human IL-28 R alpha/IFN-lambda R1 Affinity Purified PAb (25 UG)【科瑞奥博】

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Human IL-28 R alpha/IFN-lambda R1 Affinity Purified PAb (25 UG)

货号： AF5260-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF5260-SP
别名	class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 receptor subunit alpha; IL-28R1; IL28RA; IL-28RA; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2
全称	Interleukin 28 Receptor alpha
反应种属	Human
应用	Western Blot(2 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑28 R alpha is observed.
使用方法	Western Blot: 2 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	163702 (Human); 242700 (Mouse); 362628 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancer. Authors: Wongthida P, Diaz RM, Galivo F Cancer Res., 2010;70(11):4539-49. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1 Arg21-Ala228 Accession # Q8IU57
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12 (CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex (1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for IL-28/IL-28 R (7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, Q. et al. (2006) Vitam. Horm. 74:207. Meager, A. et al. (2005) Cytokine 31:109. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69. Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409. Lasfar, A. et al. (2006) Cancer Res. 66:4468. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.

参考图片

Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑28 R alpha /IFN‑ lambda R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitts lymphoma cell line was stained with Human IL‑28 R alpha /IFN‑ lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).