| 货号 | AF5245-SP |
| 别名 | follistatin-like 4; Follistatin-like protein 4; follistatin-related protein 4; FSL4; FSTL4; KIAA1061 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant mouse Follistatin-like 4/FSTL4 in direct ELISAs. Detects human, mouse, and rat Follistatin-like 4/FSTL4 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FSTL4 is observed, and less than 5% cross‑reactivity with rhFSTL5 and recombinant mouse FSTL1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23105 (Human); 320027 (Mouse); 303130 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Follistatin-like 4/FSTL4 Trp23-Val841 Accession # Q5STE3 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FSTL4 (Follistatin-like protein 4; also FSL4, SPIG1 and D/Bsp120I-1) is a likely secreted, 90 kDa (predicted) member of the follistatin gene family of TGF-beta superfamily inhibitors. It is widely expressed, being found in neurons (retinal ganglion and cerebellar Purkinje cells), cardiac muscle cells, smooth muscle cells and intestinal epithelium. Mature mouse FSTL4 is 819 amino acids (aa) in length (aa 23-841). It contains one kazal-like domain (aa 80-134), an EF-hand motif (aa 173-208), and two Ig-like domains (aa 250-336 and 340-425). There are two potential splice forms, one that shows an alternative start site at Met315, and a second that possesses a His residue substitution for aa 518-841. Full-length mature mouse FSTL4 shares 95% and 84% aa identity with rat and human FSTL4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Follistatin‑like 4/FSTL4 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Follistatin-like 4/FSTL4 in Mouse Brain. Follistatin-like 4/FSTL4 was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons in the brainstem. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in human or rat samples |
