| 货号 | AF5169-SP |
| 别名 | 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuron-specific enolase; NSE |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse Enolase 2/Neuron-specific Enolase in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2026 (Human); 13807 (Mouse); 24334 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inflammation-induced reversible switch of the neuron-specific enolase promoter from Purkinje neurons to Bergmann glia | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Enolase 2/Neuron-specific Enolase Met1-Leu434 Accession # P09104 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Enolase 2 in Human Brain. Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Enolase 2 in Human Brain. Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human/Mouse Enolase 2 by Western Blot. Western blot shows lysates of human brain, mouse brain tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Enolase 2 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Human Enolase 2/Neuron‑specific Enolase by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for Enolase 2/Neuron‑specific Enolase at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron‑specific Enolase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
