| 货号 | AF5098-SP |
| 别名 | KIAA1159; neurexophilin 3; NPH3; NPH3neurexophilin-3; NXPH3 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot |
| 目标/特异性 | Detects human, mouse, and rat Neurexophilin-3/NXPH-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neurexophilin-1/NXPH1 and recombinant rat Neurexophilin-1/NXPH1 is observed. |
| 使用方法 | Western Blot |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11248 (Human); 104079 (Mouse); 59315 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Neurexophilin-3/NXPH-3 Gln23-Gly252 Accession # AAI66603 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Neurexophilins (NXPH) are a family of neuropeptide-like secreted glycoproteins expressed in specific regions of the brain. NXPH1 is expressed mainly by cells that resemble inhibitory interneurons, while NXPH3 is found selectively in subplate-derived neurons in cortical layer 6b, granule cells in the vestibulocerebellum and Cajal-Retzius cells during development. NXPH1, 2 and 3 are tightly bound extracellular ligands of alpha-neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release. NXPH2 is not expressed in rodents, and NXPH4 does not bind alpha-neurexins. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Neurexophilin‑3/NXPH‑3 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Neurexophilin‑3/NXPH‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5098) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neurexophilin‑3/NXPH‑3 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
