| 货号 | AF4988-SP |
| 别名 | T Cell Receptor alpha Chain-V alpha 24-J alpha Q; T CellReceptor alpha ChainV alpha 24J alpha Q |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human T Cell Receptor alpha Chain-V alpha 24-J alpha Q Lys22-Pro135 Accession # ABC72391 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | V alpha 24-J alpha Q (T cell receptor alpha chain) is a 31 kDa (predicted) member of the Ig superfamily. It constitutes an invariant alpha -chain that is paired with the V beta 11 chain on human NKTINV cells, a subpopulation of NKR-P1A T cells. This TCR creates a binding site for CD1d-associated ligands that are presumed to involve glyco‑/phospholipids. The human V alpha 24V beta 11 TCR is analogous to the V alpha 14V beta 8 complex in mice. Human V alpha 24-J alpha Q is 276 amino acids in length. It contains a 21 aa signal sequence, multiple N-linked glycosylation sites, a V-type Ig-like domain (aa 36‑133) and a TCR-related sequence of unknown function (aa 142‑255). Considerable variability is noted over aa 22‑114. The closest mouse ortholog to human V alpha 24-J alpha Q shows 65% aa identity over aa 22‑135. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line, mouse spleen, rat and, mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for T Cell Receptor alpha Chain-V alpha 24-J alpha Q at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
