| 货号 | AF4848-SP |
| 别名 | CRYA1; CRYA1crystallin, alpha-1; CRYAA; crystallin, alpha A; Heat shock protein beta-4; HSPB4; HSPB4alpha-crystallin A chain; human alphaA-crystallin (CRYA1), 10HspB4 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(1 µg/mL) |
| 目标/特异性 | Detects mouse AlphaA Crystallin/CRYAA in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAB is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1409 (Human); 12954 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human AlphaA Crystallin/CRYAA Met1-Ser173 Accession # P02489 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AlphaA Crystallin (CRYAA, also known as Crystallin alpha -A chain and HspB4) is a 21‑22 kDa member of the HSP20 family of proteins. It is found in lens epithelium where it noncovalently oligomerizes with CRY-alpha B to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAA is 173 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 63‑145. Multiple posttranslational modifications may exist. The C-terminal Ser and the N-terminal MetAspVal is occasionally cleaved. There is also phosphorylation at Ser45 and 122, O-linked GlcNAc modification at Ser162, deamidation at Asn101, and acetylation at Lys70. Full‑length human CRYAA is 54% aa identical to CRY-AlphaB, and 95% aa identical to mouse CRYAA. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse AlphaA Crystallin/CRYAA by Western Blot. Western blot shows lysates of mouse eye lens tissue. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. Bands for AlphaA Crystallin/CRYAA were detected at approximately 20 - 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Detection of Mouse AlphaA Crystallin/CRYAA by Simple WesternTM. Simple Western lane view shows lysates of mouse eye lens tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaA Crystallin/CRYAA at approximately 28 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
