| 货号 | AF4839-SP |
| 别名 | BIMP3; BIMP3card-maguk protein 1; CARD-containing MAGUK protein 1; Carma 1; CARMA1; CARMA1bcl10-interacting maguk protein 3; caspase recruitment domain family, member 11; caspase recruitment domain-containing protein 11; MGC133069 | 全称 | Caspase Recruitment Domain-containing Protein 11 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat CARMA1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84433 (Human); 108723 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CARD11 Lys263-Ser442 Accession # Q9BXL7 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CARMA1 (CARD-MAGUK1; also CARD11) is a 133 kDa member of the MAGUK family of proteins. It is a cytoplasmic promoter of Bcl10 phosphorylation, and as such, regulates the effectiveness of NF-kappa B signaling. Human CARMA1 is 1154 amino acids (aa) in length and contains an N-terminal CARD region, two coiled-coil (CC) domains (aa 123‑250 and 295‑442), and a PZD, SH3 and MAGUK domain (aa 996‑1133). The CC1 and MAGUK domains position the molecule, while CC1 and CC2 mediate a necessary dimerization/oligomerization. There is one potential alternate start site seven aa upstream from the standard start site. Over aa 263-442, human CARMA1 is 98% aa identical to both mouse and canine CARMA1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat CARD11/CARMA1 by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CARD11/CARMA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4839) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CARD11/CARMA1 at approximately 133 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
