| 货号 | AF4719-SP |
| 别名 | IPP2; IPP-2; IPP2phosphatase inhibitor 2; MGC87148; phosphoprotein phosphatase; PPP1R2; protein phosphatase 1, regulatory (inhibitor) subunit 2; protein phosphatase inhibitor 2 | 全称 | Protein Phosphatase 1 Inhibitor 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat PP1 Inhibitor-2 in Western blots. In Western blots, no cross-reactivity with recombinant human PP1 Inhibitor-1 or DARPP-32 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5504 (Human); 66849 (Mouse); 192361 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PP1 Inhibitor-2 Met1-Ser205 Accession # P41236 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein Phosphatase 1 Inhibitor-2 (PP1 Inhibitor-2), also called PP1 regulatory subunit 2 (PP1R2), I2, and IPP2, binds to PP1 through an RVxF consensus sequence. The complex inactivates PP1, preventing it from dephosphorylating substrates such as glycogen phosphorylase. Unlike PP1 Inhibitor-1, the binding of PP1 Inhibitor-2 is Protein Kinase A phosphorylation-independent. Dissociation of the PP1 Inhibitor-2 complex is enhanced by phosphorylation at T72 by GSK3, ERK, or CDK. Overexpression of PP1 Inhibitor-2 in mouse heart improves cardiac function without changing organ size. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PP1 Inhibitor‑2 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP1 Inhibitor-2 at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. | |
Detection of Human PP1 Inhibitor‑2 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitts lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PP1 Inhibitor‑2 at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
