| 货号 | AF4665-SP |
| 别名 | inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2; NAALADase L2; NAALADL2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase-like 2 | 全称 | N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human NAALADase-like 2/NAALADL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse NAALADase 2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 254827 (Human); 635702 (Mouse); 499583 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human NAALADase-like 2/NAALADL2 Ser152-Asn795 (Met194Thr) Accession # CAE75743 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of NAALADase‑like 2/ NAALADL2 in LnCAP Human Cell Line by Flow Cytometry. LnCAP human prostate cancer cell line was stained with Goat Anti-Human NAALADase‑like 2/NAALADL2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4665, filled histogram) or control antibody (Catalog # |
