| 货号 | AF4567-SP |
| 别名 | casein kinase 1, epsilon; casein kinase I isoform epsilon; CKIe; CKI-epsilon; CSNK1E; EC 2.7.11; EC 2.7.11.1; HCKIE; MGC10398 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat CK1 epsilon in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha, CK1δ, or CK1 gamma. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1454 (Human); 27373 (Mouse); 58822 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CK1 epsilon Met317-Lys416 Accession # P49674 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3,δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 epsilon phosphorylates all three mammalian PER proteins, as well as BMAL1 and CRY, thereby modulating the activities of these circadian clock regulators. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Casein Kinase 1 epsilon by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitts lymphoma cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1 epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4567) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 epsilon at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
