| 货号 | AF4554-SP |
| 别名 | DKFZp434J2031; EC 3.1.3.16; FLJ10181; integrin-linked kinase-associated serine/threonine phosphatase 2C; integrin-linked kinase-associated serine/threonine phosphatase; MGC4846; PP2C delta; PP2C-DELTA; protein phosphatase 2c, delta isozyme | 全称 | Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human ILKAP in Western blots. In direct ELISAs, this antibody shows weak cross-reactivity with PP2C alpha (4%), PP2C beta (6%), and PP2C gamma (1%). |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 80895 (Human); 67444 (Mouse); 64538 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ILKAP Met1-His392 Accession # Q9H0C8 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn2+ ions but inhibited by Mg2+and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat ILKAP/PP2Cδ by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4554) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ILKAP/PP2C delta at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
