| 货号 | AF4465-SP |
| 别名 | PKC iota/lambda/zeta | 全称 | Protein Kinase C iota/lambda/zeta |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat PKC iota / lambda / zeta in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PKC iota Ile455-Val596 Accession # P41743 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their co‑factor requirements: conventional PKC isoforms ( alpha, beta Ι, beta ΙΙ, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon, eta, and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota, lambda, and zeta ), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC iota has 72% overall identity to PKC zeta. Atypical PKCs have been shown to serve as a convergent downstream target for the PI 3-kinase and TC10 signaling pathways. Stimulation of atypical PKCs by TNF-alpha has been shown to be required for NF‑ kappa B activation. Furthermore, insulin-stimulated atypical PKC activation has been directly implicated in the translocation of GLUT4 and glucose uptake in adipocytes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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PKC iota / lambda / zeta in Human Pancreas. PKC iota / lambda / zeta was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human/Mouse/Rat PKC iota / lambda / zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples. | |
Detection of Human, Mouse, and Rat PKC iota / lambda / zeta by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKC iota / lambda / zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human PKC iota, PKC lambda, and PKC zeta (2 ng/lane) were included. Specific bands were detected at approximately 22 kDa for recombinant PKC iota / lambda and ~80 kDa for natural PKC iota / lambda (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
