| 货号 | AF4419-SP |
| 别名 | 14-3-3 epsilon; 14-3-3 protein epsilon; FLJ45465; FLJ53559,14-3-3E; KCIP-1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide; YWHAE |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat 14-3-3 epsilon in Western blots. In Western blots, less than 1% cross-reactivity with other recombinant human 14-3-3 isoforms is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7531 (Human); 22627 (Mouse); 29753 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human 14-3-3 epsilon Met1-Gln255 Accession # P62258 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | 14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/ Rat 14‑3‑3 epsilon by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14‑3‑3 epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14‑3‑3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14‑3‑3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
