| 货号 | AF4376-SP |
| 别名 | C polypeptide; EC 2.3.2.13; G-ALPHA-h; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGCGNAH; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2; Transglutaminase C; Transglutaminase H; transglutaminase-2; tTG |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) |
| 目标/特异性 | Detects human Transglutaminase 2/TGM2 in direct ELISAs and human, mouse, and rat Transglutaminase 2/TGM2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TGM3, rhTGM4, and rhTGM7 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7052 (Human); 21817 (Mouse); 56083 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distinct fibroblast lineages determine dermal architecture in skin development and repair. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 2/TGM2 Ala2-Ala687 (Asn533Thr, Leu655Val) Accession # P21980 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Transglutaminase 2/TGM2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Transglutaminase 2/TGM2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line loaded at 0.2 mg/mL. A specific band was detected for Transglutaminase 2/TGM2 at approximately 82 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human/Mouse/Rat Transglutaminase 2/TGM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4376) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
