| 货号 | AF4210-SP |
| 别名 | ALK tyrosine kinase receptor; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246 antigen; CD246; EC 2.7.10.1; Ki-1; NBLST3; Tcrz | 全称 | Anaplastic Lymphoma Kinase |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ALK/CD246 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 238 (Human); 11682 (Mouse); 266802 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ALK/CD246 Val19-Ser1038 Accession # Q9UM73 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ALK (Anaplastic Lymphoma Kinase; also CD246) is a 200-220kDa member of the Insulin receptor subfamily, tyrosine kinase family, proteinkinase superfamily of proteins. It showsrestricted expression, being limited to select sympathetic and sensory neurons,endothelial cells and tumor cells. Althoughactivation of ALK appears to induce cell proliferation, the exact ligand(s) forALK is unknown. In atemporally-regulated context, midkine has been suggested to bind to ALK, andionic Zn has been reported to activate ALK cytoplasmically. Mature human ALK is a 1602 amino acid (aa) typeI transmembrane glycoprotein (SwissProt #:Q9UM73). It contains a 1020 aa extracellular region (aa19-1038) that contains one protein-protein MAM domain (aa 264-427), an LDL receptorclass A region (aa 437-473), a second MAM domain (aa 480-631) and one utilizedphosphorylation site at Ser211. This isaccompanied by a long 561 aa cytoplasmic domain that possesses a protein kinasedomain (aa 1116-1392) plus eleven utilized Tyr phosphorylation sites. There is one potential splice variant thatcontains a four aa insert after Glu549. There is also an 80 kDa soluble product that arises from cleavage of theextracellular domain. Although a third140 kDa product is frequently seen in SDS-Page, its structural basis isunclear. Almost 20 distinct ALK fusionproducts arise from gene translocations involving multiple intracellularmolecules. These seem to involve blocksof aa starting between aa 1050-1060 and continuing to aa 1620. Over aa 19-1038, human ALK shares 88% aasequence identity with mouse ALK. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ALK/CD246 by Western Blot. Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ALK/CD246 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4210) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ALK/CD246 at approximately 220 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ALK/CD246 in Human Brain. ALK/CD246 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human ALK/CD246 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4210) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
