| 货号 | AF3579-SP |
| 别名 | c-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; EC 2.7.12.2; JNK kinase 2; JNK-activating kinase 2; JNKK 2; JNKK2; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK7; MEK 7; MEK7; mitogen-activated protein kinase kinase 7; MKE7; MKK7MAPKK 7; PRKMK7 | 全称 | Mitogen-activated Protein Kinase Kinase 7 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MKK7 in Western blots. Does not cross-react with other recombinant human mitogen-activated protein kinase kinases. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5609 (Human); 26400 (Mouse); 363855 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MKK7 Met1-Arg419 Accession # O14733 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MAP Kinase Kinase 7 (MKK7), also known as MAP2K7, is a member of the dual-specificity MAP kinase kinase family with a predicted molecular weight of approximately 47.5 kDa. It is widely expressed in mammalian tissue and localizes to both the cytoplasm and the nucleus. The MKK7 protein contains three N-terminal D-sites, a central catalytic domain, and a C-terminal DVD domain. MAP kinase kinase kinases activate MKK7 via binding of the DVD domain and phosphorylation of a serine and threonine residue within the activation loop of the catalytic domain. MKK7 then utilizes its D-sites to bind and activate the JNK family of MAP kinases via phosphorylation. The genetic inactivation of MKK7 in mice results in embryonic lethality, indicating that MKK7 is an essential gene. Accordingly, MKK7 appears to be involved in the regulation of diverse physiological processes, including axon elongation, the circadian clock, embryonic stem cell differentiation, and liver regeneration. MKK7 function may also be important for the prevention of certain diseases. It has been shown to inhibit lung and mammary cancer via JNK1- and JNK2-dependent p53 stabilization. Additionally, reduced MKK7 function has been linked to the development of schizophrenia. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MKK7 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human MKK7 by Western Blot. Western blot shows recombinant human MEKs and recombinant human MKKs Catalog#s??? (??? ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
