| 货号 | AF3564-SP |
| 别名 | AADC; AADCaromatic-L-amino-acid decarboxylase; DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); DOPA decarboxylase; EC 4.1.1; EC 4.1.1.28 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(1-15 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Dopa Decarboxylase/DDC in direct ELISAs and human, mouse and rat Dopa Decarboxylase/DDC in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 1-15 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1644 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dopa Decarboxylase/DDC Met1-Glu480 Accession # P20711 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinsons disease (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Dopa Decarboxylase/DDC by Western Blot. Western blot shows lysates of mouse kidney tissue, PC‑12 rat adrenal pheochromocytoma cell line, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Dopa Decarboxylase/DDC in IMR‑32 Human Cell Line. Dopa Decarboxylase/DDC was detected in immersion fixed IMR‑32 human neuroblastoma cell line using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Dopa Decarboxylase/DDC in Human Brain. Dopa Decarboxylase/DDC was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Dopa Decarboxylase/DDC in Mouse Brain. Dopa Decarboxylase/DDC was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
