| 货号 | AF3490-SP |
| 别名 | 1-Cys; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; aiPLA21-Cys PRX; Antioxidant protein 2; AOP2; AOP2Non-selenium glutathione peroxidase; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.-; KIAA0106Red blood cells page spot 12; Liver 2D page spot 40; MGC46173; NSGPx; NSGPx1-Cys peroxiredoxin; p29; peroxiredoxin 6; peroxiredoxin-6; PRDX6; PRX |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat Peroxiredoxin 6 on in Western blots. In Western blots, this antibody does not cross-react with recombinant human Peroxiredoxin 1, 2, 3, or 5. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9588 (Human); 11758 (Mouse); 94167 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Peroxiredoxin 6 Met1-Pro224 Accession # P30041 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human peroxiredoxin-6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. The molecule is 224 amino acids (aa) in length and has but one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A2 activity. Thus, Prx-6 likely plays a role in phospholipid turnover. The enzyme effect is a function of Ser32 in a GWSWG motif. Human Prx-6 is 93%, 92%, 90%, and 94% aa identical to porcine, rat, mouse, and canine Prx-6, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Peroxiredoxin 6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
