| 货号 | AF3337-SP |
| 别名 | C1qC; C1Q-C; C1QGcomplement component 1, q subcomponent, gamma polypeptide; complement C1q subcomponent subunit C; complement component 1, q subcomponent, C chain; FLJ27103 | 全称 | Complement Component 1q Subcomponent Subunit C Precursor |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Complement C1qC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) C1qA and rhC1qB is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 714 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human C1qC Lys115-Asp245 Accession # P02747 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | C1qC is a globular secreted protein and is a subunit of complement component C1q. C1q is composed of 18 polypeptide chains of three distinct subtypes (C1qA, C1qB and C1qC) arranged as 6 disulfide-linked A-B heterodimers and 3 disulfide-linked C-C homodomers. All C1q subunits contain a C1q globular domain and one collagen-like domain. C1q is a subcomponent of complement C1 complex which comprises one C1q and the Ca2+-dependent serine protease tetramer C1r2-C1s2. C1 complex triggers the classical complement pathway. Within the C1q domain, human C1qC shares approximately 75% amino acid sequence identity with mouse and rat C1qC. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Complement Component C1qC in Human HUVEC. Complement Component C1qC was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Complement Component C1qC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3337) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
