| 货号 | AF3186-SP |
| 别名 | casein kinase 1, gamma 1; casein kinase I isoform gamma-1; CKI-gamma 1; CSNK1G1; EC 2.7.11; EC 2.7.11.1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(3-15 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat CK1 gamma in Western blots. In Western blots, the immunogen is derived from a region of human CK1 gamma that is 97% to 98% identical to human CK1 gamma 2 and CK1 gamma 3, so all three isoforms should be detected. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 3-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 53944 (Human); 214897 (Mouse); 64086 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CK1 gamma Val182-Tyr304 Accession # Q9HCP0 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, beta, gamma 1, gamma 2, gamma 3, δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. The C-termini of the gamma isoforms carry putative palmitoylation sites unique within the CK1 family that are believed to anchor the proteins in the plasma membrane. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Casein Kinase 1 gamma by Western Blot. Western blot shows lysates of human, mouse and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CK1 gamma isoforms at approximately 45 to 51 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. | |
Casein Kinase 1 gamma in Human Brain. Casein Kinase 1 gamma was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
